{"id":412,"date":"2025-10-08T10:57:15","date_gmt":"2025-10-08T10:57:15","guid":{"rendered":"https:\/\/sarkarhospital.in\/?page_id=412"},"modified":"2025-12-13T08:21:32","modified_gmt":"2025-12-13T08:21:32","slug":"zero-sperm-count-azoospermia-pesa-tesa-tese-micro-tese","status":"publish","type":"page","link":"https:\/\/sarkarhospital.in\/?page_id=412","title":{"rendered":"ZERO SPERM COUNT \u2013 AZOOSPERMIA (PESA \/ TESA \/ TESE \/ Micro TESE)"},"content":{"rendered":"\t\t
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Zero Sperm Count - Azoospermia \u2013 NIL count\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Azoospermia \u2013 Nil Report, Still Full of Hope<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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Azoospermia<\/strong> refers to the complete absence of sperm<\/strong> in the ejaculated semen. This condition naturally leads to infertility, as fertilization cannot occur without sperm. The diagnosis should always be confirmed through at least three semen analyses<\/strong> performed at a reliable laboratory<\/strong>.<\/p>

At Sarkar Hospital, Agra<\/strong>, we have extensive experience in successfully treating azoospermic men<\/strong> and helping them become fathers with their own sperm<\/strong>. Each case is approached with personalized and need-based treatment<\/strong>, combining medical, surgical, IVF\u2013ICSI, or integrated approaches<\/strong> to optimize the chances of success.<\/p>

It\u2019s important to understand that normal physical features or sexual performance<\/strong>\u2014such as facial hair growth, muscle build, or normal intercourse\u2014do not necessarily indicate normal sperm count<\/strong>. Only a microscopic semen examination<\/strong> can confirm the presence or absence of sperm.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Many men diagnosed with azoospermia can still father their own biological children<\/strong>. Once the diagnosis is confirmed, the next step is to determine the underlying cause<\/strong> and evaluate treatment options, which may include medications, surgery, or advanced assisted reproductive techniques (ART)<\/strong> such as IVF with ICSI<\/strong>.<\/p>

Even men who have undergone vasectomy (voluntary sterilization)<\/strong> can regain fertility\u2014either through microsurgical vasectomy reversal<\/strong> or by retrieving sperm directly from the testes<\/strong> for use in IVF\u2013ICSI<\/strong>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Symptoms of Azoospermia<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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Azoospermia<\/strong>, the medical condition marked by the absence of sperm in the semen<\/strong>, often shows no visible or obvious symptoms<\/strong>. Most men discover the condition only after experiencing difficulty in conceiving<\/strong>. However, certain signs or associated conditions may point toward azoospermia or underlying reproductive issues:<\/p>

  • Infertility:<\/strong> The most common indication of azoospermia is the inability to achieve pregnancy<\/strong>, even after regular unprotected intercourse.<\/p><\/li>

  • Pain or Swelling:<\/strong> Some men may experience discomfort or swelling<\/strong> in the testicles or scrotum<\/strong>, which can signal an underlying problem affecting sperm production or transport.<\/p><\/li>

  • Sexual Dysfunction:<\/strong> Issues such as erectile dysfunction<\/strong>, reduced libido<\/strong>, or low semen volume<\/strong> may be linked to hormonal imbalances<\/strong> or structural abnormalities.<\/p><\/li>

  • Hormonal Imbalances:<\/strong> Signs like gynecomastia<\/strong> (enlarged breast tissue in men), fatigue<\/strong>, or changes in muscle mass or mood<\/strong> may indicate hormonal disturbances<\/strong> that interfere with sperm production<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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    Causes of Azoospermia<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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    Azoospermia can be caused by a variety of factors, broadly categorized into obstructive and non-obstructive types:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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    1. Obstructive Azoospermia<\/strong><\/h4>

    This form of azoospermia happens when there is a physical blockage<\/strong> that prevents sperm from mixing with semen during ejaculation, even though the testes produce sperm normally. Common causes include:<\/p>

    • Vasectomy:<\/strong> A common male sterilization procedure that cuts or seals the vas deferens, stopping sperm from reaching the ejaculate.<\/p><\/li>

    • Congenital Absence of the Vas Deferens:<\/strong> A genetic condition where the vas deferens (the tube carrying sperm from the testicles) is missing from birth.<\/p><\/li>

    • Infections:<\/strong> Sexually transmitted infections (STIs) or other infections may cause inflammation and scarring<\/strong>, leading to blockage.<\/p><\/li>

    • Post-Surgical Complications:<\/strong> Prior surgeries in the scrotal or pelvic area can result in scar tissue formation and obstruction.<\/p><\/li><\/ul>

      2. Non-Obstructive Azoospermia<\/strong><\/h4>

      In this type, the issue lies in sperm production<\/strong> within the testes or due to hormonal imbalances<\/strong> that regulate fertility. Common causes include:<\/p>

      • Genetic Disorders:<\/strong> Conditions such as Klinefelter syndrome<\/strong>, Y chromosome microdeletions<\/strong>, or other genetic abnormalities can interfere with sperm formation.<\/p><\/li>

      • Hormonal Imbalances:<\/strong> Problems in the pituitary gland or hypothalamus<\/strong> can affect testosterone and other hormones essential for sperm production.<\/p><\/li>

      • Testicular Damage:<\/strong> Injury, infections like mumps orchitis<\/strong>, or testicular cancer<\/strong> can impair normal sperm development.<\/p><\/li>

      • Environmental Factors:<\/strong> Prolonged exposure to toxins, radiation, chemicals<\/strong>, or high temperatures<\/strong> can reduce or stop sperm production.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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        Diagnosis of azoospermia<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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        The diagnosis of azoospermia is made during an infertility consultation, which in men systematically includes a spermogram. This examination consists of analyzing the content of the ejaculate (semen), evaluating various parameters and comparing the results with the standards established by the WHO.<\/p>

        In the event of azoospermia, no sperm is found after centrifugation of the entire ejaculate. To make the diagnosis, however, it is necessary to perform one or even two other spermograms, each 3 months apart, because spermatogenesis (sperm production cycle) lasts about 72 days. In the absence of sperm production over 2 to 3 consecutive cycles, the diagnosis of azoospermia will be made.<\/p>

        Various additional examinations will be carried out to refine the diagnosis and try to identify the cause of azoospermia:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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        • Clinical Examination:
          A thorough physical examination is performed, including palpation of the testes to assess their consistency and size, measurement of testicular volume, and examination of the epididymis and vas deferens to detect any abnormalities or obstructions.<\/p><\/li>

        • Seminal Biochemistry:
          A biochemical analysis of semen evaluates secretions like fructose, zinc, citrate, carnitine, and acid phosphatases from glands such as the seminal vesicles, prostate, and epididymis. Alterations in these secretions can help identify the site and nature of a blockage in the reproductive tract.<\/p><\/li>

        • Hormonal Assessment:
          A blood test is done to measure key reproductive hormones, including FSH (Follicle-Stimulating Hormone), testosterone, thyroid hormones, and prolactin.<\/p>

          • High FSH levels may indicate testicular damage.<\/p><\/li>

          • Low FSH levels suggest dysfunction of the hypothalamic-pituitary axis.<\/p><\/li><\/ul><\/li>

          • Serological Testing:
            Blood tests are performed to detect infections such as Chlamydia or other pathogens that could cause inflammation or damage in the reproductive tract.<\/p><\/li>

          • Scrotal Ultrasound:
            A high-resolution ultrasound helps visualize the testes, epididymis, and vas deferens, allowing detection of structural abnormalities, cysts, or blockages.<\/p><\/li>

          • Genetic Testing and Karyotype Analysis:
            Chromosomal studies and Y-chromosome microdeletion testing may be done to identify genetic abnormalities affecting sperm production or transport.<\/p><\/li>

          • Testicular Biopsy:
            A minimally invasive procedure, performed under local anesthesia, in which a small tissue sample from the testis is examined microscopically to evaluate sperm production and testicular health.<\/p><\/li>

          • Imaging of the Pituitary Gland:
            In selected cases, an X-ray or MRI scan of the pituitary gland may be recommended if a hormonal or central cause of azoospermia is suspected.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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            Genetic testing<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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            • Genetic counselling and chromosome analysis are recommended before starting treatment. The chromosome analysis is made from a blood sample and carried out by a human geneticist. The result is available after about two weeks. Apart from checking for the normal chromosomes, micro deletion of the Y chromosome is also checked. A Z F region on the Y chromosome in male is responsible for sperm production. Detection of abnormality in this region can help us diagnose and prognosticate the treatment..<\/li>
            • A gene defect causing cystic fibrosis disease of the lung is common in patients with absent vas. The gene is studied before using the sperm from a man with absent vas to rule out this possibility.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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              Azoospermia treatment<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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              1. Hormone Deficiency<\/strong><\/h4>

              In cases where azoospermia is caused by a hormonal imbalance<\/strong>, hormone replacement or stimulation therapy can restore natural sperm production.<\/p>

              • Specific hormones are given as medicines<\/strong> to supplement or replace deficient hormones.<\/p><\/li>

              • Treatment is typically continued for a minimum of 3 months<\/strong> and monitored through regular progress evaluations.<\/p><\/li>

              • In properly selected patients, normal sperm production resumes naturally<\/strong>.<\/p><\/li>

              • Pregnancy can occur through natural intercourse<\/strong>.<\/p><\/li>

              • Sperm production may decline after stopping the therapy, but semen can be frozen<\/strong> for future use.<\/p><\/li>

              • The treatment can be repeated if needed.<\/p><\/li><\/ul>

                At Sarkar Hospital<\/strong>, we have successfully treated numerous men with hormone-related azoospermia, helping them become fathers naturally<\/strong>.<\/p>

                2. Infection<\/strong><\/h4>

                When azoospermia results from infection of the reproductive tract:<\/p>

                • Diagnosis is made through detailed history, physical examination, and semen culture<\/strong>.<\/p><\/li>

                • Drug sensitivity testing<\/strong> identifies the most effective antibiotic.<\/p><\/li>

                • A long course of targeted medication<\/strong> can completely clear the infection and restore sperm flow<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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                  Treatment by surgery<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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                  1. Vas Opening Operations<\/strong><\/h4>

                  In men with blockage of the vas deferens<\/strong>, surgery may help restore sperm transport.<\/p>

                  • The vas deferens<\/strong> is a fine tube carrying sperm from the testes to the semen.<\/p><\/li>

                  • Blockage can occur anywhere along its length, making precise diagnosis and repair challenging.<\/p><\/li>

                  • The surgery must be performed using microsurgical techniques<\/strong> under a microscope by highly skilled specialists.<\/p><\/li>

                  • Though possible, this surgery is technically demanding<\/strong>, and results may vary depending on the extent of damage.<\/p><\/li>

                  • With the excellent success of IVF\u2013ICSI<\/strong>, most patients now prefer these less invasive fertility solutions over major surgery.<\/p><\/li>

                  • However, for men with previous vasectomy<\/strong>, vas reopening surgery can still offer excellent outcomes.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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                    2. Varicocele Correction<\/strong><\/h4>

                    Varicocele is a dilation of the scrotal veins<\/strong> that can affect sperm production.<\/p>

                    • It rarely causes complete azoospermia, but in selected cases<\/strong>, microsurgical varicocele repair<\/strong> can improve sperm recovery chances.<\/p><\/li><\/ul>

                      3. Testes Fixation Surgery<\/strong><\/h4>

                      In some men, testes fail to descend fully into the scrotum<\/strong> (cryptorchidism).<\/p>

                      • Early surgical fixation<\/strong> of the testes in childhood helps preserve fertility.<\/p><\/li>

                      • Late fixation in adulthood<\/strong>, however, is less effective in restoring sperm function.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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                        IVF\u2013ICSI with Sperm from Epididymis or Testes<\/strong><\/h3>

                        If no sperm is found in semen but present in the testes or epididymis, sperm retrieval<\/strong> can be performed for use in IVF with ICSI or IMSI<\/strong>.<\/p>

                        Procedure Overview<\/strong><\/h4>

                        • Sperm is collected directly from the epididymis or testicular tissue<\/strong> through minimally invasive techniques.<\/p><\/li>

                        • Retrieved sperm or testicular tissue is cryopreserved (frozen)<\/strong> in multiple portions for future IVF cycles, minimizing the need for repeated biopsies.<\/p><\/li>

                        • On the day of egg retrieval<\/strong>, the frozen sample is thawed, and viable sperm are selected to fertilize the eggs using ICSI (Intracytoplasmic Sperm Injection)<\/strong>.<\/p><\/li>

                        • Unused samples<\/strong> remain safely stored for future use.<\/p><\/li><\/ul>

                          If no sperm are found<\/strong> during testicular examination, couples can still pursue parenthood using donor sperm<\/strong>, available through Sarkar Hospital\u2019s affiliated ART Bank<\/strong>, which maintains fully tested, high-quality donors<\/strong> in compliance with legal and ethical standards.<\/p>

                          Success Rates<\/strong><\/h4>

                          When viable sperm are retrieved, ICSI success rates<\/strong> are comparable to those achieved with normal ejaculated sperm.<\/p>

                          • Average pregnancy success rates per cycle<\/strong> range from 55% to 60%<\/strong>, depending on additional fertility factors.<\/p><\/li>

                          • The success at Sarkar Hospital<\/strong> is attributed to our highly skilled embryology team<\/strong> and state-of-the-art IVF lab facilities<\/strong>.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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                            Sperm Retrieval Techniques<\/strong><\/h3>

                            Depending on the site and likelihood of sperm presence, our fertility specialists choose the most appropriate and least invasive retrieval method. Only one side is examined initially \u2014 the other is explored only if necessary.<\/p>

                            1. PESA (Percutaneous Epididymal Sperm Aspiration)<\/strong><\/h4>

                            • A fine needle<\/strong> is inserted into the epididymis, and gentle suction<\/strong> retrieves the sperm-rich fluid.<\/p><\/li>

                            • No cuts, stitches, or dressing are required.<\/p><\/li>

                            • The patient can resume normal activities immediately<\/strong>.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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                              2. TESA (Testicular Sperm Aspiration)<\/strong><\/h4>

                              • A fine needle<\/strong> aspirates testicular tubules directly from the testis.<\/p><\/li>

                              • Multiple aspirations can be done if needed.<\/p><\/li>

                              • No incision or sutures are required.<\/p><\/li>

                              • Recovery is immediate.<\/p><\/li><\/ul>

                                3. TESE (Testicular Sperm Extraction \u2013 Biopsy)<\/strong><\/h4>

                                • A small incision<\/strong> is made to extract small tissue samples.<\/p><\/li>

                                • A single self-dissolving stitch<\/strong> is placed.<\/p><\/li>

                                • The patient can return to normal activities the next day<\/strong>.<\/p><\/li><\/ul>

                                  4. Micro-TESE (Microscopic Testicular Sperm Extraction)<\/strong><\/h4>

                                  • Conducted under spinal or general anesthesia<\/strong>.<\/p><\/li>

                                  • The testis is opened and examined under a surgical microscope<\/strong> to locate sperm-producing tubules.<\/p><\/li>

                                  • Offers the highest chance of sperm retrieval<\/strong>, especially in non-obstructive azoospermia<\/strong>.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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                                    Advantage<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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                                    • Maximum chance of locating viable sperm.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t
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                                      Disadvantage<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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                                      • It is a major operation<\/li>
                                      • It requires major anaesthesia<\/li>
                                      • It requires more exploration of the testis; hence chances of negative effect on testicular function are more.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

                                        Zero Sperm Count – Azoospermia \u2013 NIL count https:\/\/www.youtube.com\/watch?v=YFc3iL_EFXo Azoospermia \u2013 Nil Report, Still Full of Hope Azoospermia refers to […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_joinchat":[],"footnotes":""},"class_list":["post-412","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages\/412","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=412"}],"version-history":[{"count":5,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages\/412\/revisions"}],"predecessor-version":[{"id":1408,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages\/412\/revisions\/1408"}],"wp:attachment":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=412"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}