{"id":523,"date":"2025-10-24T11:51:41","date_gmt":"2025-10-24T11:51:41","guid":{"rendered":"https:\/\/sarkarhospital.in\/?page_id=523"},"modified":"2025-12-13T07:32:22","modified_gmt":"2025-12-13T07:32:22","slug":"cryopreservation","status":"publish","type":"page","link":"https:\/\/sarkarhospital.in\/?page_id=523","title":{"rendered":"CRYOPRESERVATION"},"content":{"rendered":"\t\t
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Cryopreservation \u2013 Freezing and storage<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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Cryopreservation<\/strong> is the process of freezing biological tissues<\/strong> at extremely low temperatures for future use.
Modern technology now allows the safe and effective preservation of all types of reproductive tissues<\/strong>, including:<\/p>

  • Embryos<\/strong><\/p><\/li>

  • Blastocysts<\/strong><\/p><\/li>

  • Eggs (Oocytes)<\/strong><\/p><\/li>

  • Sperms (Semen)<\/strong><\/p><\/li>

  • Testicular tissue<\/strong><\/p><\/li>

  • Ovarian tissue<\/strong><\/p><\/li><\/ul>

    The main goal of cryopreservation is to preserve fertility potential<\/strong> by keeping these tissues biologically active and healthy for later use in assisted reproduction.<\/p>

    How Cryopreservation Works<\/strong><\/h2>

    Reproductive tissues are stored in liquid nitrogen<\/strong> at a temperature of \u2013196\u00b0C<\/strong>.
    At this ultra-low temperature, all cellular activity stops completely<\/strong>, placing the cells in a suspended state of life<\/strong>.
    This allows them to remain viable for years or even decades<\/strong>, without any damage to their biological function.<\/p>

    At Sarkar Hospital, Agra<\/strong>, we use advanced vitrification technology<\/strong> \u2014 the latest and most reliable freezing method \u2014 to ensure excellent survival and pregnancy rates after thawing.<\/p>

    Our Expertise and Global Training<\/strong><\/h2>

    Our fertility team has received specialized training<\/strong> under Dr. Tetsunori Mukaida<\/strong> and the expert team of the Hiroshima HART Institute, Japan<\/strong>.
    Dr. Mukaida is one of the pioneers and inventors of the vitrification technique<\/strong>.<\/p>

    This international collaboration has enabled us to bring world-class cryopreservation standards<\/strong> to Agra, ensuring superior outcomes and patient safety<\/strong>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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    Techniques<\/h3>\t\t\t\t<\/div>\n\t\t\t\t
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    1. The Challenge of Freezing Cells<\/strong><\/h3>

    Human body cells contain a high amount of water.
    During freezing, this water can form ice crystals<\/strong>, which may damage cell structure<\/strong> and reduce viability after thawing.
    This problem is more significant with larger cells<\/strong>, such as eggs (oocytes)<\/strong> and embryos<\/strong>.
    To overcome this, two advanced techniques are used \u2014 Vitrification<\/strong> and Slow Freezing<\/strong>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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    2. Vitrification (Fast Freezing)<\/strong><\/h3>

    Vitrification is an ultra-rapid freezing process<\/strong>.
    The cells are cooled so quickly that ice crystals do not have time to form<\/strong>.
    Instead, the cell fluid transforms into a glass-like or jelly-like state<\/strong>, protecting the delicate cell structure.<\/p>

    Benefits of Vitrification:<\/strong><\/p>

    • Excellent survival rate<\/strong> after thawing<\/p><\/li>

    • High fertilization and implantation success<\/strong><\/p><\/li>

    • Preferred for embryos and oocytes<\/strong> due to their large size and sensitivity<\/p><\/li><\/ul>

      This is the preferred technique<\/strong> at Sarkar Hospital<\/strong> for freezing eggs and embryos<\/strong>, ensuring the highest possible success rates in future IVF cycles.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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      3. Slow Freezing<\/strong><\/h3>

      In this method, the tissue temperature is gradually lowered<\/strong> in a programmed, computer-controlled freezer<\/strong>.
      It allows the water inside cells to move out slowly, minimizing ice formation.<\/p>

      Preferred for:<\/strong><\/p>

      • Sperm samples<\/strong><\/p><\/li>

      • Testicular tissue<\/strong><\/p><\/li>

      • Ovarian tissue<\/strong><\/p><\/li><\/ul>

        Though it takes longer than vitrification, slow freezing<\/strong> is highly effective for these tissue types.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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        Embryo - Blastocyst Cryopreservation<\/h4>\t\t\t\t<\/div>\n\t\t\t\t
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        Cryopreservation technology allows embryos, blastocysts, eggs, and sperms<\/strong> to be frozen safely and used later \u2014 without compromising success rates<\/strong>.
        Thanks to advanced vitrification techniques<\/strong>, frozen embryos today offer the same chances of pregnancy<\/strong> as fresh embryo transfers.<\/p>

        This means couples can achieve their dream of parenthood without undergoing ovarian stimulation or egg retrieval again<\/strong>, saving both time and cost<\/strong> \u2014 a frozen embryo transfer typically costs less than one-third<\/strong> of a fresh IVF cycle.<\/p>

        How Long Can Embryos Be Preserved?<\/strong><\/h2>

        Embryos or blastocysts can be safely stored for up to ten years or even longer<\/strong> without affecting their quality or success potential.<\/p>

        When is Embryo or Blastocyst Cryopreservation Done?<\/strong><\/h2>

        Embryo freezing is recommended in several situations:<\/p>

        • When extra embryos<\/strong> are available after a fresh IVF cycle.<\/p><\/li>

        • When fresh embryo transfer is not possible<\/strong> due to:<\/p>

          • Ovarian hyperstimulation (OHSS)<\/p><\/li>

          • Endometrial or hormonal imbalance<\/p><\/li>

          • Any other medical condition<\/p><\/li><\/ul><\/li>

          • When couples want to delay pregnancy<\/strong> but face declining fertility (e.g., women over 35, poor ovarian reserve, before cancer treatment, or ovarian surgery).<\/p><\/li>

          • As part of repeated IVF failure strategy<\/strong>, to optimize results.<\/p><\/li>

          • When ERA (Endometrial Receptivity Array)<\/strong> test shows the uterus is not ready for implantation.<\/p><\/li>

          • For poor ovarian responders<\/strong>, to accumulate embryos from multiple IVF cycles before PGT (Pre-implantation Genetic Testing)<\/strong>.<\/p><\/li>

          • As part of Dual Stimulation (DuoStim)<\/strong> protocols to improve outcomes in patients with low ovarian reserve.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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            Oocyte cryopreservation \u2013 Egg freezing Vitrification<\/h5>\t\t\t\t<\/div>\n\t\t\t\t
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            Your Fertility, Your Control<\/strong><\/h3>

            Egg freezing empowers women to preserve their fertility<\/strong> and plan motherhood at their convenience.
            For women who wish to delay pregnancy<\/strong>, do not yet have a partner<\/strong>, or prefer not to create embryos, oocyte vitrification<\/strong> is the ideal option.<\/p>

            The goal is to freeze eggs at their healthiest stage<\/strong>, maintaining the same chances of pregnancy later as at the time of freezing.<\/p>

            At Sarkar Hospital, Agra<\/strong>, we use the most advanced vitrification technology<\/strong> to ensure excellent survival and fertilization rates after thawing.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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            When is Egg Freezing Recommended?<\/strong><\/h3>

            Egg freezing is beneficial for:<\/p>

            • Women planning motherhood beyond 35 years of age<\/strong><\/p><\/li>

            • Women with reduced ovarian function<\/strong> or irregular cycles who wish to delay pregnancy<\/p><\/li>

            • Cancer patients<\/strong> before chemotherapy, radiotherapy, or bone marrow transplantation<\/p><\/li>

            • Non-cancer patients<\/strong> undergoing treatments toxic to the ovaries<\/p><\/li>

            • Women needing major ovarian or endometriosis surgery<\/strong> that may reduce ovarian reserve<\/p><\/li><\/ul>

              In all these cases, cryopreservation offers a safe and effective way to protect fertility<\/strong> for future use.<\/p>

              Process of Egg Freezing<\/strong><\/h3>

              The process is similar to IVF up to the stage of egg retrieval:<\/p>

              1. Ovarian Stimulation<\/strong> \u2013 Hormonal injections (FSH, HMG) are given to produce multiple eggs.<\/p><\/li>

              2. Egg Retrieval<\/strong> \u2013 Once follicles mature, eggs are collected under mild anesthesia using ultrasound guidance.<\/p><\/li>

              3. Assessment & Freezing<\/strong> \u2013 Mature eggs are evaluated and then frozen using vitrification<\/strong>, which prevents ice crystal formation.<\/p><\/li>

              4. Storage & Future Use<\/strong> \u2013 The eggs can remain frozen indefinitely. When ready for use, they are thawed, fertilized<\/strong>, and the resulting embryos are transferred to the uterus<\/strong> just like standard IVF.<\/p><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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                Semen \u2013 sperm cryopreservation \u2013 semen freezing<\/h5>\t\t\t\t<\/div>\n\t\t\t\t
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                Sperm freezing is a simple, safe, and affordable<\/strong> procedure that preserves male fertility effectively.<\/p>

                When is Sperm Freezing Recommended?<\/strong><\/h3>

                • When the husband cannot be present<\/strong> on the day of egg retrieval<\/p><\/li>

                • Men with travel-intensive jobs<\/strong>, military duties, or living abroad<\/p><\/li>

                • Men who have difficulty producing semen on demand<\/strong><\/p><\/li>

                • Before IVF to ensure backup sperm availability<\/strong><\/p><\/li>

                • Men with very low sperm count<\/strong> or deteriorating sperm quality<\/p><\/li>

                • Sperm obtained surgically<\/strong> from testis or epididymis<\/p><\/li>

                • Cancer patients<\/strong> before chemotherapy or radiotherapy<\/p><\/li>

                • Non-cancer patients<\/strong> undergoing treatments toxic to sperms<\/p><\/li>

                • Sperm donation programs<\/strong>, where frozen donor samples are kept ready in ART banks<\/p><\/li><\/ul>

                  Using frozen sperm<\/strong> for IVF gives the same success rates<\/strong> as fresh samples. For IUI, a slight reduction<\/strong> in success may occur, but results remain excellent overall.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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                  Scientific Significance of Cryopreservation<\/strong><\/h2>

                  Freezing of eggs, embryos, blastocysts, sperm, and reproductive tissues<\/strong> is now a refined and proven science<\/strong>.
                  It has revolutionized fertility preservation and improved IVF success rates<\/strong> dramatically by offering flexibility, safety, and control to both patients and doctors.<\/p>

                  Cryopreservation at Sarkar Hospital, Agra<\/strong><\/h2>

                  At Sarkar Hospital<\/strong>, we are proud to offer a comprehensive and advanced cryopreservation program<\/strong>, ensuring the highest standards of care and safety<\/strong> for every sample.<\/p>

                  Our Advantages:<\/strong><\/p>

                  • Robust freezing and storage program<\/strong> with international-grade technology<\/p><\/li>

                  • Specialized vitrification techniques<\/strong> for superior survival and pregnancy rates<\/p><\/li>

                  • Highly experienced embryologists<\/strong> trained in Japan\u2019s HART Institute vitrification protocols<\/p><\/li>

                  • Safe, long-term storage<\/strong> at \u2013196\u00b0C in liquid nitrogen<\/p><\/li>

                  • Personalized fertility preservation plans<\/strong> for every patient<\/p><\/li><\/ul>

                    We are proud to help individuals and couples secure their fertility today for a confident tomorrow<\/strong>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

                    Cryopreservation \u2013 Freezing and storage Cryopreservation is the process of freezing biological tissues at extremely low temperatures for future use.Modern […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_joinchat":[],"footnotes":""},"class_list":["post-523","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages\/523","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=523"}],"version-history":[{"count":11,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages\/523\/revisions"}],"predecessor-version":[{"id":1358,"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=\/wp\/v2\/pages\/523\/revisions\/1358"}],"wp:attachment":[{"href":"https:\/\/sarkarhospital.in\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}